female lab technician in googles

Biomarker Lab Capabilities

Flow Cytometry and Cell-based Assays

PPD® Laboratories’ biomarker lab has access to industry-leading instrumentation in both the bioanalytical and central labs. The biomarker lab can accommodate everything from research assays to fully validated assays with CAP/CLIA, GLP and GCLP compliance. The lab also provides training to clinical sites on proper sample processing for complex procedures, such as phospho-flow and activation assays. The biomarker lab has experience with various sample types including whole, frozen and lysed blood, as well as peripheral blood mononuclear cells (PBMCs). The test menu includes routine cell surface assays and intracellular assays.

Key instrumentation:

BD FACSCanto™ II flow cytometers

  • Nine flow cytometry systems with multi-parameter analysis capability
  • Three lasers and 10 detectors per system
  • Accurate results for experiments requiring up to 10 parameters

Ligand-binding Assays

PPD Laboratories’ biomarker lab offers traditional ELISA, electrochemiluminescence and microfluidics-based ELISA. We work in consultation with customers to develop a tiered approach to validation depending on the needs of the program or phase of development of the biomarker.

Our biomarker experts are in close contact with the various lab locations to ensure integration and a smooth transition of assays and technology during development of the biomarker program.

Key instrumentation:

  • MSD®
  • ELISA
  • ProteinSimple ELLA
  • GYROS
  • Luminex

Immunohistochemistry

Our biomarker lab leverages PPD Laboratories’ strategic alliance with NeoGenomics for immunohistochemistry (IHC) services.

Liquid Chromatography/Mass Spectrometry

The biomarker lab is equipped with exceptional liquid chromatography/mass spectrometry (LC-MS/MS) instrumentation to support biomarker method development and validation. Our experienced scientists, with knowledge of the current perspectives in biomarker assay validation, work with customers to develop a customized solution, which includes novel and routine biomarker assays. We maintain multiple LC-MS technologies, including triple quadrapoles, HPLC, UHPLC and multi-dimensional chromatography.

Key instrumentation:

  • Hamilton® MICROLAB® STARlet automatic pipetting workstations
  • Sciex Triple Quad LC-MS systems – 4000, 5000, 5500, 6500
  • Shimadzu Nexera UHPLC systems with 2-D capabilities
  • Waters Acquity UPLC systems with 2-D capabilities
  • Waters Xevo® G3 QTof (HRMS)
  • Thermo Scientific UHPLC systems with 3-D and nanoflow capabilities
  • Thermo Scientific Aria® multiplexing systems
  • Thermo Scientific Quantiva Triple Quadrupole LC-MS systems
  • Thermo Scientific Q Exactive Plus (HRMS)

Molecular Genomics

PPD Laboratories has a custom-built, spacious, leading-edge molecular genomics suite in Richmond, Virginia. The suite of labs is designed with the appropriate engineering and procedural controls to minimize the risk of reagent and sample contamination.

It includes:

  • Restricted access main entrance
  • Central gowning laboratory that services the main suite
  • Directional airflow and pressure control
  • 100% exhaust biosafety cabinets
  • Full (clean room style) gowning
  • Whole room UV decontamination system
  • Interlocked portals for transport of samples in and out of the room
  • Industry-leading environmental monitoring system

Key instrumentation:

  • Agilent Mx3005P qPCR System
  • Agilent TapeStation 2200 Bioanalyzer
  • Applied Biosystems® 3730xl Genetic Analyzer
  • Applied Biosystems 7500
  • Applied Biosystems QuantStudio 6
  • BioMérieux NucliSENS® easyMag® nucleic acid extraction system
  • BioRad Gel Doc XR+ Imaging System
  • BioRad QX200 Droplet Digital PCR (ddPCR™) System
  • Illumina® MiSeq (NextGen sequencer)
  • Qiagen BioRobot Universal System
  • Qiagen QIAsymphony® SP nucleic acid extraction system
  • TECAN EVO® automated liquid-handling

Take a virtual tour of our genomics laboratory

BIOMARKER LAB EXPERTS

Related Resources

Learn more about PPD Laboratories’ biomarker lab capabilities.